Narrowband Filters for Enzyme Labeling Instruments
Material and Construction: Hard coatings are deposited on substrates made of colored glass bonded with BK7, equivalent to high-quality glass sourced from Hoya.
Optical Density: Standard Optical Density (OD) is ≥OD4, ensuring effective blocking of unwanted wavelengths.
Bandwidth: The standard bandwidth is 10nm +/- 1nm, allowing sharp isolation of specific wavelengths.
Customization: Besides standard configurations, parameters such as center wavelength, bandwidth, and optical density can be tailored to match the spectral characteristics of target analytes.
Applications of ELISA Readers: DNA and RNA Quantification and Purity Analysis (A260/A280), Protein Quantification (A280/BCA/Bradford/Lowry), Enzyme Activity and Kinetics Detection, Cell Proliferation and Toxicity Analysis, Cell Apoptosis Detection, Reporter Gene Detection, G Protein-Coupled Receptor (GPCR) Assays, etc.
Enzyme Labeling Instruments, commonly known as Enzyme-linked Immunosorbent Assay (ELISA) readers, are specialized devices used for enzyme-linked immunosorbent testing. These instruments utilize colorimetric methods to analyze the content of antigens or antibodies, providing critical data for various biochemical and clinical applications. The precise operation of ELISA readers relies on narrow bandpass filters to achieve accurate monochromatic light for detecting enzymatic reactions.
Specifications:
| Coating Type | Hard Coating |
| Full Width-Half Max (FWHM) | 10.0+/-1nm |
| Minimum Transmission | >50% |
| Optical Density (OD) | ≧4.0 |
| Block Wavelength Range | 200 ~1200nm |
| Surface Quality | 80/50 S/D |
| Substrate Materials | Colored Glass + BK7 |
Key Features:
- Material and Construction: Hard coatings are deposited on substrates made of colored glass bonded with BK7, equivalent to high-quality glass sourced from Hoya.
- Optical Density: Standard Optical Density (OD) is ≥OD4, ensuring effective blocking of unwanted wavelengths.
- Bandwidth: The standard bandwidth is 10nm +/- 1nm, allowing sharp isolation of specific wavelengths.
- Customization: Besides standard configurations, parameters such as center wavelength, bandwidth, and optical density can be tailored to match the spectral characteristics of target analytes.
Applications of ELISA Readers:
ELISA readers are extensively used for:
- DNA and RNA Quantification and Purity Analysis (A260/A280)
- Protein Quantification (A280/BCA/Bradford/Lowry)
- Enzyme Activity and Kinetics Detection
- Cell Proliferation and Toxicity Analysis
- Cell Apoptosis Detection
- Reporter Gene Detection
- G Protein-Coupled Receptor (GPCR) Assays
Kingwin Optics’ narrow bandpass filters are indispensable for enhancing the performance of enzyme labeling instruments. Their high transmission efficiency, precise wavelength isolation, and customizable options make them ideal for accurate and reliable enzyme-linked immunosorbent assays, helping laboratories and research facilities achieve superior analytical results.
